The accessibility of PCR and qPCR systems has increased to where they have almost considered a commodity product today. This is certainly the case for end-point PCR systems and some qPCR units also allowing researchers to carry out gene expression analysis more conveniently and quickly. This accessibility has been an innovative development in the community and will continue to move research forward for years to come.
There have been concerns with regards to reverse transcriptase (RT-PCR) and real-time RT-PCR analysis of sensitive sample types such as RNA due to the stability of the sample during process and storage. Just how much impact this has had on the results may go unknown. Recent advances in techniques such as digital PCR and new advances in extraction stabilization systems have sought to address some of these concerns.
Read the entire article, Considerations for Extraction, Stabilization and Quantification by PCR for Sensitive Sample Such as RNA, by Andrew J. Birnie, Ph.D., Global Life Sciences Product Manager, Cole-Parmer, that delves into PCR techniques, extraction techniques and a sample prep kit that extracts DNA in 3 seconds!
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