Cole-Parmer in Research: Reproducing in vivo metabolic environments in cancer research

Cancer Research

Cole-Parmer in Research: Cancer Research

The internal physiological environment of cells within the body is very complex. In cancer research it is important to ensure that studies reflect the in vivo situation of tumours to make models representative of reality. In a recent study, Voorde et al (2019), demonstrated that the nutrient content of commercially sourced media can result in “metabolic artefacts on cancer cells”.

An untargeted metabolic comparison investigated how triple-negative breast cancer (TNBC) cultured cells were affected by a nutrient-rich culture medium in comparison to a standard commercial media in vitro. The TNBC cells were seeded in media in Techne Cell Culturing Flasks to create spheres for Liquid Chromatography Tandem Mass Spectrometry analysis. In vivo results were also collected for comparison. A Principal component analysis grouped the cell cultures by biological conditions and displayed that nutrient content influences grouping.

This research has highlighted the importance of trace elements in cancer biology. Indeed, it has determined that altering nutrients, to reflect those in the bloodstream, improves the integrity of in vivo models of tumour behaviour in relation to those conducted in vitro.

Author: Dr J J Lock, EMEA Cole-Parmer

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