How to Optimize qPCR Assays for Rapid and Reproducible Results
Date: Friday, March 19, 2021
Time: 10 am PST (Los Angeles) | 12 pm CST (Chicago) | 1 pm EST (New York)
- Joe Griffith-Barrasso
- Guest Speaker Dr. Stephen Bustin, Professor of Molecular Medicine at Anglia Ruskin University
- Madalina Enache, Product Sales Manager, Antylia Scientific (EMEA)
Time: 60 mins (40 mins presentation, 20 mins Q&A)
Real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been indispensable in the fight against COVID-19. However, there happens to be numerous falsehoods, misconceptions, assumptions, and exaggerated expectations with regards to its capabilities, performance, and usefulness.
PCR today is an essential tool for countless industries and research applications. Utilizing the biological DNA replication machinery, PCR technology amplifies small fragments of genetic material to facilitate downstream analysis and processes.
This molecular technique was recently thrust into the limelight when the World Health Organization (WHO) declared the outbreak of COVID-19 a pandemic, and PCR testing became the preferred technology for detection of the disease-causing virus SARS-CoV-2.
It has also been leveraged in other aspects of disease control and prevention; screening for variants and environmental monitoring are just a few ways scientists have used PCR to combat this global crisis. It is, therefore, essential that the true strengths and limitations of PCR are restated in the context of the current COVID-19 pandemic.
Over the course of the last twelve months, demand for PCR-based techniques and PCR capabilities has skyrocketed. So, whether you are an experienced scientist or a newbie in the lab, learning how to optimize your PCR assays and multiplexing protocols could benefit both your productivity in the lab and the quality of your results.
Antylia Scientific™ (formerly Cole-Parmer) offers a range of innovative tools that can be leveraged for rapid, reliable, and reproducible PCR analysis.
In this webinar you will learn:
- About the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines
- How to optimize your qPCR and multiplexing protocols for rapid and reliable results
- How to leverage technology to improve your PCR analysis efficiency
About the presenters
Dr. Stephen Bustin – Professor of Molecular Medicine at Anglia Ruskin University
Stephen Bustin is Professor of Molecular Medicine at Anglia Ruskin University in the UK, where his research interests center around developing novel approaches for the early diagnosis of infectious diseases. He has authored numerous papers, review articles, and book chapters aimed at improving the reproducibility and robustness of molecular methods, especially those based on the use of PCR and has presented hundreds of talks and workshops worldwide on this subject. He has published three books, the “A-Z of Quantitative PCR” (2004), “The PCR Revolution” (2011) and “PCR Technology” (2013). Professor Bustin was an expert witness advising the UK High Court and the US Department of Justice on PCR technology in the Measles Mumps Rubella (MMR) vaccine – Autism class action as well as at the MMR trial in Washington DC in 2007, respectively, and has acted as an expert witness in several international patent and criminal trials. He led an international consortium developing the MIQE guidelines for the use and reporting of real-time (2009) and digital PCR (2013). Professor Bustin has extensive editorial involvements as Editor-in Chief (Gene Expression), International Journal of Molecular Sciences, and as a member of the editorial boards of several peer-reviewed journals. He acts as a consultant to advise a number of UK-based and international companies.
Madalina Enache – Product Sales Manager (EMEA) – PCRmax®, Techne®, and Argos Technologies®
Madalina holds a BSc (with Hons) in Biology and a MSc in Microbiology. She joined Antylia Scientific™ three years ago, when it was known as Cole-Parmer, as part of the application sales team. She has risen through several commercial roles, focused on PCR products, to become the EMEA Product Sales Manager for our PCRmax, Techne, and Argos Technologies brands.