A deeper understanding of chromatography fundamentals can enrich your performance
Presented by : Patricia Atkins – Senior Applications Scientist, SPEX® CertiPrep and Alan H. Katz, PhD – Director, Global Product Manager – Chemicals
Chromatography is a simple concept wrapped up in a complex set of processes, chemistries, and instrumentation, which work together to produce accurate results for both detection and separation objectives. The choice of each component, chemical, and column affect the outcome and efficiency of analysis. By breaking down the function of each key area and component, we can gain a deeper understanding of the process, making method development and validation easier to achieve. Chromatography is a critical tool that analytical scientists use every day, often without much consideration for how all the parts work together. If you work in an analytical lab, you may routinely run the same methods, without regard for process improvement; equipment choices or small adjustments to variables that may greatly enhance accuracy and efficiency.
Chemical selection for the solid and mobile phases is also critical for any successful separation; but sometimes such method development pose a challenge leading to a costly trial and error approach. With a deeper understanding of the nature and chemistries of the different columns and solvents, scientist can approach method development and validation in an organized fashion.
With the advent of time, the portfolio of available liquid chromatography detectors has increased exponentially. New techniques continue to enter the market and inevitably become integrated into the laboratory protocols. With a strong knowledge of the fundamental principles, lab personnel can identify the technologies that will lead to increased productivity, with more confidence.
Whether you are involved in daily sample processing activities, such as sample preparation, or the building and running of columns, in charge of purchasing equipment, or tasked with stocking reagents and consumables, a deeper understanding of chromatography fundamentals will enrich your performance within your designated roles.
In this webinar, we will take a deeper look into the history, operation, and functionality of liquid chromatography to show analyst how the different components fit together to perform their function. From separation to solvent chemistry, we will break down the processes needed to make liquid chromatography work for the lab.
In this webinar you will:
- Learn how to optimize chromatographic systems, taking into consideration variables such as wavelength, polarity, and pH.
- Explore how different components of modern HPLC systems fit into analytical lab methodologies and gain a deeper understanding of its process and function.
- Learn about the relationship between UV-VIS detection in HPLC and spectroscopy, using a new line of internal standards, specially created for measuring very low sample concentrations in dynamic assays.
About the presenters:
Patricia Atkins – Senior Applications Scientist at SPEX CertiPrep
Patricia graduated from Rutgers University in NJ and started her career as a laboratory supervisor for Ciba Specialty Chemicals. In 2008, Patricia joined SPEX® CertiPrep within their Certified Reference Material’s division. As a senior application scientist, she spends much of her time researching industry trends and developing new reference materials. She is an experienced speaker and has presented at numerous conferences, including NACRW, NEMC, Pittcon and AOAC. She is also a published author and her work has appeared in various journals and trade publications, including Spectroscopy, LCGC and Cannabis Science and Technology, where she is a columnist for analytical issues in botanicals testing.
Dr. Alan Katz, PhD – Director, Global Product Manager – Chemicals
Alan holds a B.A. in Chemistry from the University at Buffalo and a Ph.D. from Princeton University. His specialties include organic, computational, heterocyclic, and synthetic chemistry, drug discovery, biological assay design, structure-based molecular modeling, and lab automation. He has enjoyed combining his expertise with his interest in computer programming to develop a system to assist in drug discovery. At SPEX CertiPrep, he oversees the manufacturing and quality control of certified chemical reference materials, including inorganic and organic chemical standards, NPD and R&D.