{"id":13827,"date":"2021-11-30T13:00:21","date_gmt":"2021-11-30T19:00:21","guid":{"rendered":"https:\/\/www.coleparmer.com\/blog\/?p=13827"},"modified":"2022-11-14T08:28:30","modified_gmt":"2022-11-14T14:28:30","slug":"reach-perfect-peaks-in-chromatography","status":"publish","type":"post","link":"https:\/\/www.coleparmer.com\/blog\/reach-perfect-peaks-in-chromatography\/","title":{"rendered":"How to Reach Perfect Peaks in Your Chromatography"},"content":{"rendered":"<h2>Use mobile phase modifiers to add acids to your HPLC and LC\/MS systems.<\/h2>\n<p>High Performance Liquid Chromatography (HPLC) is one of the most common instrumental techniques for analyzing mixtures of organic compounds in analytical labs around the world. HPLC is a complex process that depends on each molecule\u2019s ability to interact with the mobile phase that carries it through the column, the solid phase that fills the columns, as well as interactions between the different molecules in the sample.<\/p>\n<p>These interactions are particularly important in LC\/MS when the molecules being analyzed need ionizable functionality. Are you using reverse-phase high performance chromatography (RP-HPLC) where the stationary phase is hydrophobic or nonpolar? Many labs are using this type of chromatography today. However, reverse-phase columns may contain open silanol groups, which may interact with polar molecules. This leads to reduced resolution, tailing of peaks, and reduction in column life.<\/p>\n<h2>Modifiers<\/h2>\n<p>The addition of modifiers, like trifluoracetic, acetic and formic acid, and the corresponding ammonium salts, is widely used to improve the quality of RP-HPLC chromatography analyses. These modifiers adjust the pH of the mobile phase and provide you with the ability to control the level of ionization of each molecule.<\/p>\n<h2>Mobile phase modifiers<\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-13833 alignleft\" src=\"https:\/\/www.coleparmer.com\/blog\/wp-content\/uploads\/2021\/11\/Spex-Mobile-Phase-Modifiers-2.jpg\" alt=\"Spex Mobile Phase Modifiers\" width=\"292\" height=\"252\">With mobile phase modifiers, you have a safe and easy way to add acetic, formic and trifluoroacetic acid to HPLC and LC\/MS systems. The new Spex Mobile Phase Modifiers allow you to simply crack a glass ampule and pour the precise amount of modifier into the mobile phase of your chromatography analyses. These premeasured, sealed ampules reduce contamination and safety risk, as well as reduce measurement inaccuracies.<\/p>\n<p>You no longer need to create, measure, and dispense hazardous chemicals, a process that causes exposure to harmful chemicals and creates inconsistency in the analyses because acids and bases degrade quickly and are prone to contamination. Now with these handy glass ampules, you can add premeasured modifiers into your mobile phase safely and accurately.<\/p>\n<p><a href=\"https:\/\/www.spex.com\/Product\/Index\/Mobile-Phase-Modifiers\/a10d3ca1-47ca-4a00-ba6b-1a5d448d9bef\" rel=\"nofollow noopener noreferrer\" target=\"_blank\">Spex mobile phase modifiers<\/a> are conveniently packaged for error-free use and designed to be directly added to the mobile-phase reservoirs for easy use. You can mix and match modifiers to create personalized method development kits.<\/p>\n<p><a href=\"https:\/\/www.spex.com\/getmedia\/b60ede75-26ed-48f5-bf86-6d6b83a8fc9a\/Optimizing-Your-Mobile-Phase_Importance-of-Solvent-and-Modifier.pdf?ext=.pdf\" rel=\"nofollow noopener noreferrer\" target=\"_blank\">Learn more<\/a> about liquid chromatography and modifiers.<\/p>\n<p>\u00a0<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-12323\" src=\"https:\/\/www.coleparmer.com\/blog\/wp-content\/uploads\/2020\/10\/CP_MA_Bottom_Blog-reminder-for-blog.jpg\" alt=\"Sign up for the blog banner\" width=\"600\" height=\"100\"><\/p>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Use mobile phase modifiers to add acids to your HPLC and LC\/MS systems. High Performance Liquid Chromatography (HPLC) is one of the most common instrumental techniques for analyzing mixtures of organic compounds in analytical labs&#8230;<\/p>\n","protected":false},"author":20,"featured_media":13834,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-13827","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.7 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>How to Reach Perfect Peaks in your Chromatography<\/title>\n<meta name=\"description\" content=\"Here&#039;s a safe and easy way to add acetic, formic and trifluoroacetic acid to HPLC and LC\/MS systems to reach perfect peaks.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link 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