pH<\/b><\/b><\/a><\/p>\nQ: I need a pH electrode to measure many things in my lab including TRIS buffers. Which one should I go with?<\/p>\n
A: TRIS buffers can contaminate or clog some probes, so choosing the correct electrode is important for these applications.\u00a0In the past a specialized Calomel electrode was used.\u00a0 However, the internal reference pin in the electrode was made with Hg+, and due to mercury restrictions, these electrodes are no longer preferred.\u00a0Instead any double junction electrode can be used.\u00a0Single junction electrodes will be susceptible to clogging.\u00a0The double junction has two layers of protection, which will ensure a decent life span of your probe even when measuring TRIS buffers.<\/p>\n
Baths and Circulators<\/p>\n
Q: I want to use a bath for a circulation loop application.\u00a0Will my pump work?<\/p>\n
A: To figure this out, we need to know: 1) How much heating or cooling energy your loop will need and 2) How much back pressure your system will be required to overcome<\/p>\n
Both of these points are important. For 1) Equations can be used to approximate the amount of energy you need to maintain or heat your process and an error factor is applied to ensure that your unit has the ability.\u00a0For 2) Most circulators include one or two pumps for circulation.\u00a0If the unit only has the force pump, it may not be powerful enough.\u00a0This pump is designed to maintain temperature uniformity, but may have some ability to circulate in open systems.\u00a0 An additional pump called a suction pump may be required, as it will provide considerable more pressure capabilities.\u00a0Not all force\/suction pumps are the same so care should be taken to ensure that the pressure output of the circulator.<\/p>\n
Mixers<\/b><\/p>\n
Q: My mixer isn\u2019t performing well, do you have a larger propeller?<\/p>\n
A: We offer a wide selection of impellers, but let\u2019s make sure you need one. How is your mixer set up?\u00a0 What type of container do you have?\u00a0Many poor mixing issues can be solved by adjusting the angle and placement of the mixer.\u00a0The best angle varies from tank to tank but is usually around 15-20\u00ba offset.\u00a0Straight up and down can cause vortexing, which is good for dispersion in light fluids but not as good for other mixing applications.\u00a0A cylinder tank also is not ideal for mixing, but is most commonly used.\u00a0If a square or rectangular container is available it may provide the mixing you are looking for.<\/p>\n
Also, we need to be careful how much strain you put on your mixer.\u00a0The motor on your mixer is only rated for so much torque.\u00a0If chosen improperly, a small increase in diameter of your impeller can add an exponential load and wear out your motor.<\/p>\n","protected":false},"excerpt":{"rendered":"
Our tech experts respond to some of your most-asked questions, covering pH, baths and circulators, and mixers. pH Q: I need a pH electrode to measure many things in my lab including TRIS buffers. Which…<\/p>\n","protected":false},"author":20,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[13,16],"tags":[],"class_list":["post-3438","post","type-post","status-publish","format-standard","hentry","category-for-the-lab","category-ph-2"],"yoast_head":"\n
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