{"id":3912,"date":"2014-06-05T10:00:07","date_gmt":"2014-06-05T15:00:07","guid":{"rendered":"https:\/\/www.coleparmer.com\/blog\/?p=3912"},"modified":"2019-09-24T11:21:56","modified_gmt":"2019-09-24T17:21:56","slug":"working-with-human-pathogens-consider-these-types-of-biological-safety-cabinets","status":"publish","type":"post","link":"https:\/\/www.coleparmer.com\/blog\/working-with-human-pathogens-consider-these-types-of-biological-safety-cabinets\/","title":{"rendered":"Working with Human Pathogens? Consider These Types of Biological Safety Cabinets"},"content":{"rendered":"<p><a href=\"http:\/\/www.nsf.org\/regulatory\/regulator-nsf-standards\/\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">NSF\u00ae<\/a> (formerly the National Sanitation Foundation) has specified three classes of biological safety cabinets that offer effective containment for working with human pathogens. Class I, II, and III are described below, and some may include sub class levels.<a href=\"https:\/\/www.coleparmer.com\/blog\/wp-content\/uploads\/2014\/06\/3-ft-purifier-cell-logic-class-ii-a2-115v-scope-ready.gif\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-thumbnail wp-image-3914\" src=\"https:\/\/www.coleparmer.com\/blog\/wp-content\/uploads\/2014\/06\/3-ft-purifier-cell-logic-class-ii-a2-115v-scope-ready.gif?w=105\" alt=\"3-ft-Purifier-Cell-Logic+,-Class-II-A2,-115V,-Scope-Ready-\" width=\"105\" height=\"150\"><\/a><\/p>\n<p><a href=\"https:\/\/www.coleparmer.com\/Category\/Biosafety_Cabinets_Class_II\/44351?referred_id=5618\" target=\"_blank\" rel=\"nofollow noopener noreferrer\"><strong>Biosafety Cabinets:<\/strong><\/a><\/p>\n<p><strong>Class I:<\/strong> Unrecirculated air flows away from the lab worker. After going through a HEPA filter, this airflow is vented into the room. This class provides the lab worker a medium level of protection but does not protect the samples in the cabinet from contamination. Essentially, this level offers staff and environmental protection, but no product protection.<\/p>\n<p><strong>Class II:<\/strong> This level is best if a lab worker is using microorganisms in containment at level 2, 3, and 4 labs. These cabinets are divided into two types (A and B) determined by construction, airflow velocities, patterns, and exhaust systems. The Class II cabinets protect the lab worker, samples, and the external environment.<\/p>\n<p><strong>Class III:<\/strong> This level offers maximum protection for staff workers and the environment as well as samples and is designed to work with biosafety level 4 microbiological agents. These biosafety cabinets have both a HEPA filtered supply and exhaust air and are completely gas-tight and enclosed. Tests are performed while using long-sleeved gloves. The cabinet is under negative pressure of at least 120 Pa while the exterior exhaust system maintains airflow.<\/p>\n<p><em>View our selection of <\/em><a href=\"https:\/\/www.coleparmer.com\/Category\/Biosafety_Cabinets_Class_II\/44351?referred_id=5618\" target=\"_blank\" rel=\"nofollow noopener noreferrer\"><em>biosafety cabinets<\/em><\/a><em> and other <\/em><a href=\"https:\/\/www.coleparmer.com\/Category\/Fume_Hoods\/1885?referred_id=5618\" target=\"_blank\" rel=\"nofollow noopener noreferrer\"><em>fume hoods<\/em><\/a><em>.<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>NSF\u00ae (formerly the National Sanitation Foundation) has specified three classes of biological safety cabinets that offer effective containment for working with human pathogens. Class I, II, and III are described below, and some may include&#8230;<\/p>\n","protected":false},"author":20,"featured_media":3914,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[13,19],"tags":[168,172],"class_list":["post-3912","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-for-the-lab","category-safety-tips","tag-biological-safety-cabinets","tag-biosafety-cabinets"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.7 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Working with Human Pathogens? 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Class I, II, and III are described below, and some may include...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.coleparmer.ca\/blog\/working-with-human-pathogens-consider-these-types-of-biological-safety-cabinets\/\" \/>\n<meta property=\"og:site_name\" content=\"Cole-Parmer Blog\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/antylia\" \/>\n<meta property=\"article:published_time\" content=\"2014-06-05T15:00:07+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2019-09-24T17:21:56+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/i0.wp.com\/www.coleparmer.ca\/blog\/wp-content\/uploads\/2014\/06\/3-ft-purifier-cell-logic-class-ii-a2-115v-scope-ready.gif?fit=210%2C300&ssl=1\" \/>\n\t<meta property=\"og:image:width\" content=\"210\" \/>\n\t<meta property=\"og:image:height\" content=\"300\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/gif\" \/>\n<meta name=\"author\" content=\"Cole-Parmer Blog Team\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@antylia\" \/>\n<meta name=\"twitter:site\" content=\"@antylia\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Cole-Parmer Blog Team\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Working with Human Pathogens? 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Class I, II, and III are described below, and some may include...","og_url":"https:\/\/www.coleparmer.ca\/blog\/working-with-human-pathogens-consider-these-types-of-biological-safety-cabinets\/","og_site_name":"Cole-Parmer Blog","article_publisher":"https:\/\/www.facebook.com\/antylia","article_published_time":"2014-06-05T15:00:07+00:00","article_modified_time":"2019-09-24T17:21:56+00:00","og_image":[{"width":210,"height":300,"url":"https:\/\/i0.wp.com\/www.coleparmer.ca\/blog\/wp-content\/uploads\/2014\/06\/3-ft-purifier-cell-logic-class-ii-a2-115v-scope-ready.gif?fit=210%2C300&ssl=1","type":"image\/gif"}],"author":"Cole-Parmer Blog Team","twitter_card":"summary_large_image","twitter_creator":"@antylia","twitter_site":"@antylia","twitter_misc":{"Written by":"Cole-Parmer Blog Team","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.coleparmer.ca\/blog\/working-with-human-pathogens-consider-these-types-of-biological-safety-cabinets\/#article","isPartOf":{"@id":"https:\/\/www.coleparmer.ca\/blog\/working-with-human-pathogens-consider-these-types-of-biological-safety-cabinets\/"},"author":{"name":"Cole-Parmer Blog Team","@id":"https:\/\/www.coleparmer.com\/blog\/#\/schema\/person\/bd8d004d4d77d6b31800fa11177c6da9"},"headline":"Working with Human Pathogens? 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