{"id":7337,"date":"2017-12-01T08:51:42","date_gmt":"2017-12-01T14:51:42","guid":{"rendered":"https:\/\/www.coleparmer.com\/blog\/?p=7337"},"modified":"2019-01-30T18:47:20","modified_gmt":"2019-01-31T00:47:20","slug":"gene-expression-analysis","status":"publish","type":"post","link":"https:\/\/www.coleparmer.com\/blog\/gene-expression-analysis\/","title":{"rendered":"Considerations for Extraction, Stabilization and Quantification by PCR for Sensitive Sample"},"content":{"rendered":"<p>The accessibility of <a href=\"\/applications\/pcr\" rel=\"nofollow noopener noreferrer\" target=\"_blank\">PCR and qPCR systems<\/a> has increased to where they have almost considered a commodity product today. This is certainly the case for end-point <a href=\"\/c\/thermal-cyclers\" rel=\"nofollow noopener noreferrer\" target=\"_blank\">PCR systems<\/a> and some <a href=\"\/i\/pcrmax-eco-48-real-time-qpcr-system-100-to-240-v-50-60-hz\/9394700\" rel=\"nofollow noopener noreferrer\" target=\"_blank\">qPCR units<\/a> also allowing researchers to carry out gene expression analysis more conveniently and quickly. This accessibility has been an innovative development in the community and will continue to move research forward for years to come.<\/p>\n<p>There have been concerns with regards to reverse transcriptase (RT-PCR) and real-time RT-PCR analysis of sensitive sample types such as RNA due to the stability of the sample during process and storage. Just how much impact this has had on the results may go unknown. Recent advances in techniques such as digital PCR and new advances in extraction stabilization systems have sought to address some of these concerns.<\/p>\n<p>Read the entire\u00a0article, <a href=\"\/tech-article\/pcr-considerations-for-gene-expression-analysis\" rel=\"nofollow noopener noreferrer\" target=\"_blank\">Considerations for Extraction, Stabilization and Quantification by PCR for Sensitive Sample Such as RNA<\/a>,by\u00a0Andrew J. Birnie, Ph.D., Global Life Sciences Product Manager, Cole-Parmer, that delves into PCR techniques, extraction techniques and a <a href=\"https:\/\/www.coleparmer.com\/p\/sample-prep-kit\/67891\" rel=\"nofollow noopener noreferrer\" target=\"_blank\">sample prep kit<\/a> that extracts DNA in 3 seconds!<\/p>\n<p>Browse our complete offering of <a href=\"\/c\/electrophoresis-products\" rel=\"nofollow noopener noreferrer\" target=\"_blank\">electrophoresis products.<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The accessibility of PCR and qPCR systems has increased to where they have almost considered a commodity product today. This is certainly the case for end-point PCR systems and some qPCR units also allowing researchers&#8230;<\/p>\n","protected":false},"author":20,"featured_media":7341,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1964],"tags":[],"class_list":["post-7337","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-pcr"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.7 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>PCR and Extraction Techniques for Gene Expression Analysis from Cole-Parmer<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PCR and Extraction Techniques for Gene Expression Analysis from Cole-Parmer\" \/>\n<meta property=\"og:description\" content=\"The accessibility of PCR and qPCR systems has increased to where they have almost considered a commodity product today. This is certainly the case for end-point PCR systems and some qPCR units also allowing researchers...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/\" \/>\n<meta property=\"og:site_name\" content=\"Cole-Parmer Blog\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/antylia\" \/>\n<meta property=\"article:published_time\" content=\"2017-12-01T14:51:42+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2019-01-31T00:47:20+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/i0.wp.com\/www.coleparmer.fr\/blog\/wp-content\/uploads\/2017\/11\/IMG_0016-lightened.jpg?fit=333%2C500&ssl=1\" \/>\n\t<meta property=\"og:image:width\" content=\"333\" \/>\n\t<meta property=\"og:image:height\" content=\"500\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Cole-Parmer Blog Team\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@antylia\" \/>\n<meta name=\"twitter:site\" content=\"@antylia\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Cole-Parmer Blog Team\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"PCR and Extraction Techniques for Gene Expression Analysis from Cole-Parmer","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/","og_locale":"en_US","og_type":"article","og_title":"PCR and Extraction Techniques for Gene Expression Analysis from Cole-Parmer","og_description":"The accessibility of PCR and qPCR systems has increased to where they have almost considered a commodity product today. This is certainly the case for end-point PCR systems and some qPCR units also allowing researchers...","og_url":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/","og_site_name":"Cole-Parmer Blog","article_publisher":"https:\/\/www.facebook.com\/antylia","article_published_time":"2017-12-01T14:51:42+00:00","article_modified_time":"2019-01-31T00:47:20+00:00","og_image":[{"width":333,"height":500,"url":"https:\/\/i0.wp.com\/www.coleparmer.fr\/blog\/wp-content\/uploads\/2017\/11\/IMG_0016-lightened.jpg?fit=333%2C500&ssl=1","type":"image\/jpeg"}],"author":"Cole-Parmer Blog Team","twitter_card":"summary_large_image","twitter_creator":"@antylia","twitter_site":"@antylia","twitter_misc":{"Written by":"Cole-Parmer Blog Team","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/#article","isPartOf":{"@id":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/"},"author":{"name":"Cole-Parmer Blog Team","@id":"https:\/\/www.coleparmer.com\/blog\/#\/schema\/person\/bd8d004d4d77d6b31800fa11177c6da9"},"headline":"Considerations for Extraction, Stabilization and Quantification by PCR for Sensitive Sample","datePublished":"2017-12-01T14:51:42+00:00","dateModified":"2019-01-31T00:47:20+00:00","mainEntityOfPage":{"@id":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/"},"wordCount":199,"commentCount":0,"publisher":{"@id":"https:\/\/www.coleparmer.com\/blog\/#organization"},"image":{"@id":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/#primaryimage"},"thumbnailUrl":"https:\/\/www.coleparmer.com\/blog\/wp-content\/uploads\/2017\/11\/IMG_0016-lightened.jpg","articleSection":["PCR"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/","url":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/","name":"PCR and Extraction Techniques for Gene Expression Analysis from Cole-Parmer","isPartOf":{"@id":"https:\/\/www.coleparmer.com\/blog\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/#primaryimage"},"image":{"@id":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/#primaryimage"},"thumbnailUrl":"https:\/\/www.coleparmer.com\/blog\/wp-content\/uploads\/2017\/11\/IMG_0016-lightened.jpg","datePublished":"2017-12-01T14:51:42+00:00","dateModified":"2019-01-31T00:47:20+00:00","breadcrumb":{"@id":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/#primaryimage","url":"https:\/\/www.coleparmer.com\/blog\/wp-content\/uploads\/2017\/11\/IMG_0016-lightened.jpg","contentUrl":"https:\/\/www.coleparmer.com\/blog\/wp-content\/uploads\/2017\/11\/IMG_0016-lightened.jpg","width":333,"height":500,"caption":"gene expression analysis"},{"@type":"BreadcrumbList","@id":"https:\/\/www.coleparmer.fr\/blog\/gene-expression-analysis\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.coleparmer.com\/blog\/"},{"@type":"ListItem","position":2,"name":"Considerations for Extraction, Stabilization and Quantification by PCR for Sensitive Sample"}]},{"@type":"WebSite","@id":"https:\/\/www.coleparmer.com\/blog\/#website","url":"https:\/\/www.coleparmer.com\/blog\/","name":"Cole-Parmer Blog","description":"","publisher":{"@id":"https:\/\/www.coleparmer.com\/blog\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.coleparmer.com\/blog\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.coleparmer.com\/blog\/#organization","name":"Antylia Scientific","url":"https:\/\/www.coleparmer.com\/blog\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.coleparmer.com\/blog\/#\/schema\/logo\/image\/","url":"https:\/\/i0.wp.com\/www.coleparmer.co.uk\/blog\/wp-content\/uploads\/2021\/02\/AntyliaScientific_CMYK.jpg?fit=400%2C107&ssl=1","contentUrl":"https:\/\/i0.wp.com\/www.coleparmer.co.uk\/blog\/wp-content\/uploads\/2021\/02\/AntyliaScientific_CMYK.jpg?fit=400%2C107&ssl=1","width":400,"height":107,"caption":"Antylia Scientific"},"image":{"@id":"https:\/\/www.coleparmer.com\/blog\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/antylia","https:\/\/x.com\/antylia","https:\/\/www.linkedin.com\/company\/antylia\/","https:\/\/www.youtube.com\/channel\/UC41i86hGEGhQhM1KMqrDnZg"]},{"@type":"Person","@id":"https:\/\/www.coleparmer.com\/blog\/#\/schema\/person\/bd8d004d4d77d6b31800fa11177c6da9","name":"Cole-Parmer Blog Team","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.coleparmer.com\/blog\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/34e3e6aa661d0e443fb9050a9175a092?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/34e3e6aa661d0e443fb9050a9175a092?s=96&d=mm&r=g","caption":"Cole-Parmer Blog Team"},"url":"https:\/\/www.coleparmer.com\/blog\/author\/donna-donahue\/"}]}},"_links":{"self":[{"href":"https:\/\/www.coleparmer.com\/blog\/wp-json\/wp\/v2\/posts\/7337","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.coleparmer.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.coleparmer.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.coleparmer.com\/blog\/wp-json\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/www.coleparmer.com\/blog\/wp-json\/wp\/v2\/comments?post=7337"}],"version-history":[{"count":0,"href":"https:\/\/www.coleparmer.com\/blog\/wp-json\/wp\/v2\/posts\/7337\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.coleparmer.com\/blog\/wp-json\/wp\/v2\/media\/7341"}],"wp:attachment":[{"href":"https:\/\/www.coleparmer.com\/blog\/wp-json\/wp\/v2\/media?parent=7337"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.coleparmer.com\/blog\/wp-json\/wp\/v2\/categories?post=7337"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.coleparmer.com\/blog\/wp-json\/wp\/v2\/tags?post=7337"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}