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Use size exclusion method to quickly and efficiently separate high molecular weight substances such as proteins and biomolecules from salts and other small molecules. Buffer exchange or desalting of a sample can be used to prepare for mass spectroscopy analysis or lyophilization, and after certain procedures such as ion exchange chromatography. Useful alternative to dialysis for larger or degradable samples. The molecular weight cutoff is 5000. Recommended flow rate is 1 mL/min for 1-mL column, 5 mL/min for 5-mL column. Stable over pH 2 to 12 working range.
Columns feature biocompatible polypropylene body and polyethylene top and bottom filters. Each column comes with a plug in the inlet and a snap-off end at the outlet. Easily connect to a syringe, pump, or chromatography system using fingertight fittings (coned 10-32) for 1/16" OD tubing. Connect up to five columns in series for larger sample volumes.
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