The best possible transfection results are obtained by using the original buffers from Eppendorf®, which have been tested for sterility as well as for the absence of mycoplasma, endotoxins, and pyrogens, and have undergone a thorough cytotoxicity test.
The hypo-osmolar buffer is for electroporation, and increases transfection efficiency, particularly for mammalian cells. It allows the electrode formation of the cells and, as a result, easier membrane penetration. The sodium-free buffer system is adapted to the inner cell environment and stabilizes the Na+/K+ gradient across the cell membrane, so even highly sophisticated transfection experiments are possible.
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