Spex CertiPrep SALKIT-2-250 Two-Color Salmonella qPCR Detection Kit; 250 Reactions

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Spex CertiPrep Item # EW-61165-99
Rapid and accurate test with high specificity to determine the presence of Salmonella DNA
  • Measure two most common strains: enterica and bongori
  • Sensitive detection kit is capable of amplifying < 100 copies of Salmonella DNA
  • Internal control prevents false negative results
  • Compatible with any qPCR system that supports two color channels

$840.00USD / Each

SPECS & DESCRIPTION

Specifications & Description

  • Test TypeqPCR
  • Number of Tests Included250
  • Mastermix IncludedYes
  • Genus Detectedenterica and bongori
  • Binomial NameSalmonella
  • DescriptionTwo-Color Salmonella qPCR Detection Kit; 250 Reactions

Includes

2.5 mL Master Mix, 0.12 mL positive control, 0.25 mL internal control, 1 mL yellow dye, ROX, 2 mL water, and Supplier Certificate of Analysis (CoA) with product shipment.

Key Features

  • Measure two most common strains: enterica and bongori
  • Sensitive detection kit is capable of amplifying < 100 copies of Salmonella DNA
  • Internal control prevents false negative results
  • Compatible with any qPCR system that supports two color channels

More About this Item

This Spex CertiPrep® two-color Salmonella qPCR detection kit provides a rapid and accurate test to determine the presence of Salmonella DNA in a sample. The kit uses quantitative PCR (qPCR) to measure the total amount of the two most common strains of Salmonella: enterica and bongori.

The primers and probes target a Salmonella specific gene that is essential for its pathogenicity. The probe activates FAM channel, giving the user a positive result if Salmonella is present. An internal control primer and probes are also included that confirm that the PCR Master Mix is working optimally by ensuring the sample is free of PCR inhibitors that would lead to false negative results. To help distinguish the Master Mix from the template DNA and from the final product during sample preparation, an inert yellow dye is provided to label the template prior to adding to the Master Mix which contains an inert blue dye. When mixed the resulting solution is green, confirming the presence of all components. The kit works with any qPCR system that supports the two-color channels.

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