Prepacked columns provide a quick and convenient purification of polyhistidine-tagged proteins
High binding capacity and purity
Columns precharged with different metals or uncharged to accept ion of your choice
Quickly and easily purify his-tagged proteins using BabyBio IMAC columns. Columns are packed with WorkBeads™ agarose media with chelating ligands based on tris(2-aminoethyl)amine (TREN), iminodiacetic acid (IDA), or nitrilotriacetic acid (NTA). Select from uncharged TREN, or charged or uncharged IDA and NTA. Charged media contains IDA or NDA with Ni, Co, Cu, or Zn ions, which are immobilized on the media via the chelating ligand. Each column has a dynamic binding capacity of 50 mg/mL of medium. Connect columns in series for larger sample volumes.
Order BabyBio screening kits to conveniently repeat the same purification procedure with different metals to reduce the binding of host cell proteins or to optimize purity and yield. Each kit comes with four columns prepacked with WorkBeads IDA or WorkBeads NTA media precharged with Ni2+, Co2+, Cu2+, or Zn2+ ions.
Columns feature biocompatible polypropylene body and polyethylene top and bottom filters. Stable ove pH 7 to 9 range short term, 2 to 12 cleaning (stripped column). Each column comes with a plug in the inlet and a snap-off end at the outlet. Easily connect to a syringe, pump, or chromatography system using fingertight fittings (coned 10-32) for 1/16" OD tubing.