Reagents and standards are manufactured according to Standard Methods 5210B for 5-day BOD Testing. Solutions conform to regulations regarding fresh solutions. When mixing BOD dilution water add 1 mL of each solution to 1 liter of water.

Kit includes one bottle of 500mL for each: Calcium Chloride Solution, 2.75%, Ferric Chloride Solution, 0.025%, Magnesium Sulfate Solution, 2.25%, and Phosphate Buffer Solution, pH 7.2